Clostridial Genetics Short Course

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Clostridia are fascinating anaerobic microbes with important roles in human health, biotechnology and sustainability. Historically, these microbes have been notoriously difficult to genetically engineer, posing a major challenge to unlocking their potential. In recent years, synthetic biology tools for Clostridia have drastically improved, and we believe these should not be limited to specialist labs. The goal of this workshop is to provide hands-on training to early-career researchers in Clostridial genetic manipulation, to enable them to incorporate genetics in their own projects. 

In an intensive week-long workshop at Northeastern University in downtown Boston, attendees will learn the key principles and methodologies in anaerobic genetics, working with model Clostridia under the tutelage of three expert faculty. Travel, lodging and registration fees are covered by NSF support.

Wet-lab techniques include:

• Preparation of anaerobic competent cells
• Anaerobic electroporation and conjugation
• Detection and quantification of oxygen-independent reporter proteins
• Extraction of DNA from Gram positives, and colony PCR
• Tips and tricks to improve transformation efficiency

Bioinformatic approaches include:

• Shuttle vector design using Gibson and GoldenGate assembly
• Analysis of restriction/modification systems via Nanopore sequencing
• Design of CRISPR/Cas counterselection systems

These activities will be interspersed with seminars from leading experts in Clostridial research, a poster session for trainees to discuss their research and form connections, as well as excursions into Boston.

Our state-of-the-art facilities at Northeastern's Boston campus are equipped with the latest technology for anaerobic microbiology. Trainees will learn the proper use of all equipment needed for genetic engineering.

The course is designed for scientists with experience working with anaerobic microbes, who would benefit from incorporating genetic engineering into their research. As such, prior experience with anaerobic cultivation is expected. While hands-on molecular biology experience is not required, students should be familiar with the theory of standard techniques (PCR, restriction/ligation, etc.). If you are unsure about your qualifications, please get in touch with program staff via the link below.